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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | RAB5A, member RAS oncogene family |
| Gene Symbol: | RAB5A |
| Synonyms: | RAB5 |
| Gene ID: | 5868 |
| UniProt ID: | P20339 |
| Clone ID: | 24GB4525 |
| Immunogen: | A synthesized peptide derived from human Rab5 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables GDP binding activity; GTP binding activity; and GTPase activity. Involved in several processes, including amyloid-beta clearance by transcytosis; early endosome to late endosome transport; and regulation of exocytosis. Located in several cellular components, including cytoplasmic side of early endosome membrane; nucleoplasm; and terminal bouton.
Images
 | Western blotting analysis using anti-RAB5A antibody (Cat#63208). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAB5A antibody (Cat#63208, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-RAB5A antibody (Cat#63208). RAB5A expression in wild-type (WT) and RAB5A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RAB5A antibody (Cat#63208, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of RAB5A expression in HepG2 cells using anti-RAB5A antibody (Cat#63208, 1:2,000). Green, isotype control; red, RAB5A. |
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