Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | receptor for activated C kinase 1 |
| Gene Symbol: | RACK1 |
| Synonyms: | H12.3; HLC-7; PIG21; GNB2L1; Gnb2-rs1 |
| Gene ID: | 10399 |
| UniProt ID: | P63244 |
| Clone ID: | 23GB2355 |
| Immunogen: | A synthesized peptide derived from human RACK1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables several functions, including cyclin binding activity; enzyme binding activity; and protein domain specific binding activity. Involved in several processes, including regulation of protein metabolic process; regulation of signal transduction; and regulation of vesicle-mediated transport. Located in several cellular components, including midbody; perinuclear region of cytoplasm; and phagocytic cup. Part of IRE1-RACK1-PP2A complex.
Images
 | Western blotting analysis using anti-RACK1 antibody (Cat#61248). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RACK1 antibody (Cat#61248, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-RACK1 antibody (Cat#61248). RACK1 expression in wild-type (WT) and RACK1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RACK1 antibody (Cat#61248, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of RACK1 expression in HepG2 cells using RACK1 antibody (Cat#61248, 1:2,000). Green, isotype control; red, RACK1. |
 | Immunocytochemical staining of HepG2 cells with RACK1 antibody (Cat#61248, 1:1,000). Nuclei were stained blue with DAPI; RACK1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
You may also be interested in:
