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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | RAD21 cohesin complex component |
| Gene Symbol: | RAD21 |
| Synonyms: | MGS; HR21; MCD1; NXP1; SCC1; CDLS4; hHR21; HRAD21 |
| Gene ID: | 5885 |
| UniProt ID: | O60216 |
| Clone ID: | 24GB1390 |
| Immunogen: | A synthesized peptide derived from human RAD21 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is highly similar to the gene product of Schizosaccharomyces pombe rad21, a gene involved in the repair of DNA double-strand breaks, as well as in chromatid cohesion during mitosis. This protein is a nuclear phospho-protein, which becomes hyperphosphorylated in cell cycle M phase. The highly regulated association of this protein with mitotic chromatin specifically at the centromere region suggests its role in sister chromatid cohesion in mitotic cells.
Images
 | Western blotting analysis using anti-RAD21 antibody (Cat#62556). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAD21 antibody (Cat#62556, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-RAD21 antibody (Cat#62556). RAD21 expression in wild type (WT) and RAD21 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-RAD21 antibody (Cat#62556, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of RAD21 expression in HepG2 cells using anti-RAD21 antibody (Cat#62556, 1:2,000). Green, isotype control; red, RAD21. |
 | Immunocytochemical staining of HepG2 cells with anti-RAD21 antibody (Cat#62556, 1:1,000). Nuclei were stained blue with DAPI; RAD21 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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