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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ralA binding protein 1 |
| Gene Symbol: | RALBP1 |
| Synonyms: | RIP1; RLIP1; RLIP76 |
| Gene ID: | 10928 |
| UniProt ID: | Q15311 |
| Clone ID: | 24GB875 |
| Immunogen: | A synthesized peptide derived from human RALBP1 |
| Dilution: | WB 1:2,000-1:10,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
RALBP1 plays a role in receptor-mediated endocytosis and is a downstream effector of the small GTP-binding protein RAL (see RALA; MIM 179550). Small G proteins, such as RAL, have GDP-bound inactive and GTP-bound active forms, which shift from the inactive to the active state through the action of RALGDS (MIM 601619), which in turn is activated by RAS (see HRAS; MIM 190020) (summary by Feig, 2003 [PubMed 12888294]).
Images
 | Western blotting analysis using anti-RALBP1 antibody (Cat#62445). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RALBP1 antibody (Cat#62445, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-RALBP1 antibody (Cat#62445). RALBP1 expression in wild type (WT) and RALBP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-RALBP1 antibody (Cat#62445, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of RALBP1 expression in HeLa cells using RALBP1 antibody (Cat#62445, 1:2,000). Green, isotype control; red, RALBP1. |
 | Immunocytochemical staining of Hela cells with anti-RALBP1 antibody (Cat#62445, 1:1,000). Nuclei were stained blue with DAPI; RALBP1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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