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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Oligoclonal antibody |
| Gene Name: | RALY heterogeneous nuclear ribonucleoprotein |
| Gene Symbol: | RALY |
| Synonyms: | P542; HNRPCL2 |
| Gene ID: | 22913 |
| UniProt ID: | Q9UKM9 |
| Immunogen: | Recombinant protein of human RALY |
| Dilution: | WB 1:500-1:2,500; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the heterogeneous nuclear ribonucleoprotein (hnRNP) gene family. This protein may play a role in pre-mRNA splicing and in embryonic development. Alternate splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-RALY antibody (Cat#65291). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RALY antibody (Cat#65291, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-RALY antibody (Cat#65291). RALY expression in wild-type (WT) and RALY knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RALY antibody (Cat#65291, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of RALY expression in HepG2 cells using anti-RALY antibody (Cat#65291, 1:2,000). Green, isotype control; red, RALY. |
 | Immunocytochemical staining of HepG2 cells with anti-RALY antibody (Cat#65291, 1:1,000). Nuclei were stained blue with DAPI; RALY was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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