Your shopping cart is empty!
| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | RAP1B, member of RAS oncogene family |
| Gene Symbol: | RAP1B |
| Synonyms: | K-REV; RAL1B; THC11 |
| Gene ID: | 5908 |
| UniProt ID: | P61224 |
| Clone ID: | 25GB210 |
| Immunogen: | A synthesized peptide derived from human RAP1A/B |
| Dilution: | WB 1:1,000-1:5,000; FC 1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the RAS-like small GTP-binding protein superfamily. Members of this family regulate multiple cellular processes including cell adhesion and growth and differentiation. This protein localizes to cellular membranes and has been shown to regulate integrin-mediated cell signaling. This protein also plays a role in regulating outside-in signaling in platelets. Alternate splicing results in multiple transcript variants. Pseudogenes of this gene are found on chromosomes 3, 5, 6 and 9.
Images
 | Western blotting analysis using anti-RAP1B antibody (Cat#65217). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RAP1B antibody (Cat#65217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-RAP1B antibody (Cat#65217). RAP1B expression in wild-type (WT) and RAP1B shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RAP1B antibody (Cat#65217, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of RAP1B expression in HepG2 cells using anti-RAP1B antibody (Cat#65217, 1:2,000). Green, isotype control; red, RAP1B. |
You may also be interested in:
