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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 kappa |
| Clonality: | Monoclonal antibody |
| Gene Name: | ring-box 1 |
| Gene Symbol: | RBX1 |
| Synonyms: | ROC1; RNF75; BA554C12.1 |
| Gene ID: | 9978 |
| UniProt ID: | P62877 |
| Clone ID: | 24GB7170 |
| Immunogen: | Recombinant protein of human Rbx1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This locus encodes a RING finger-like domain-containing protein. The encoded protein interacts with cullin proteins and likely plays a role in ubiquitination processes necessary for cell cycle progression. This protein may also affect protein turnover. Related pseudogenes exist on chromosomes 2 and 5.
Images
 | Western blotting analysis using anti-ring-box 1 antibody (Cat#63745). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ring-box 1 antibody (Cat#63745, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-ring-box 1 antibody (Cat#63745). Ring-box 1 expression in wild-type (WT) and ring-box 1 (RBX1) shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ring-box 1 antibody (Cat#63745, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Ring-box 1 expression in C2C12 cells using anti-Ring-box 1 antibody (Cat#63745, 1:2,000). Green, isotype control; red, Ring-box 1. |
 | Immunocytochemical staining of C2C12 cells with anti-Ring-box 1 antibody (Cat#63745, 1:1,000). Nuclei were stained blue with DAPI;Ring-box 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain:High. Scale bar, 20 μm. |
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