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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ribosomal protein S6 kinase B2 |
| Gene Symbol: | RPS6KB2 |
| Synonyms: | KLS; SRK; S6K2; S6KB; STK14B; S6KI(2); S6Kbeta; p70S6Kb; P70-beta; S6K-beta2; P70-beta-1; P70-beta-2; p70(S6K)-beta |
| Gene ID: | 6199 |
| UniProt ID: | Q9UBS0 |
| Clone ID: | 24GB1235 |
| Immunogen: | A synthesized peptide derived from human P70 S6 Kinase beta |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains a kinase catalytic domain and phosphorylates the S6 ribosomal protein and eukaryotic translation initiation factor 4B (eIF4B). Phosphorylation of S6 leads to an increase in protein synthesis and cell proliferation.
Images
 | Western blotting analysis using anti-P70 S6 Kinase beta antibody (Cat#62521). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-R70 S6 Kinase beta antibody (Cat#62521, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-P70 S6 Kinase beta antibody (Cat#62521). P70 S6 Kinase beta expression in wild type (WT) and P70 S6 Kinase beta shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-P70 S6 Kinase beta antibody (Cat#62521, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of P70 S6 Kinase beta expression in HeLa cells using P70 S6 Kinase beta antibody (Cat#62521, 1:2,000). Green, isotype control; red, P70 S6 Kinase beta. |
 | Immunocytochemical staining of HeLa cells with anti-P70 S6 Kinase beta antibody (Cat#62521, 1:1,000). Nuclei were stained blue with DAPI; P70 S6 Kinase beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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