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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | structural maintenance of chromosomes 3 |
| Gene Symbol: | SMC3 |
| Synonyms: | BAM; BMH; HCAP; CDLS3; CSPG6; SMC3L1 |
| Gene ID: | 9126 |
| UniProt ID: | Q9UQE7 |
| Clone ID: | 24GB2155 |
| Immunogen: | A synthesized peptide derived from human SMC3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene belongs to the SMC3 subfamily of SMC proteins. The encoded protein occurs in certain cell types as either an intracellular, nuclear protein or a secreted protein. The nuclear form, known as structural maintenance of chromosomes 3, is a component of the multimeric cohesin complex that holds together sister chromatids during mitosis, enabling proper chromosome segregation. Post-translational modification of the encoded protein by the addition of chondroitin sulfate chains gives rise to the secreted proteoglycan bamacan, an abundant basement membrane protein.
Images
 | Western blotting analysis using anti-SMC3 antibody (Cat#62709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMC3 antibody (Cat#62709, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-SMC3 antibody (Cat#62709). SMC3 expression in wild type (WT) and SMC3 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-SMC3 antibody (Cat#62709, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of SMC3 expression in HepG2 cells using anti-SMC3 antibody (Cat#62709, 1:2,000). Green, isotype control; red, SMC3. |
 | Immunocytochemical staining of HepG2 cells with anti-SMC3 antibody (Cat#62709, 1:1,000). Nuclei were stained blue with DAPI; SMC3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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