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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | structural maintenance of chromosomes 4 |
| Gene Symbol: | SMC4 |
| Synonyms: | CAPC; CAP-C; SMC-4; SMC4L1 |
| Gene ID: | 10051 |
| UniProt ID: | Q9NTJ3 |
| Clone ID: | 24GB2400 |
| Immunogen: | A synthesized peptide derived from human SMC4 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene belongs to the 'structural maintenance of chromosomes' (SMC) gene family. Members of this gene family play a role in two changes in chromosome structure during mitotic segregation of chromosomes- chromosome condensation and sister chromatid cohesion. The protein encoded by this gene is likely a subunit of the 13S condensin complex, which is involved in chromosome condensation. A pseudogene related to this gene is located on chromosome 2.
Images
 | Western blotting analysis using anti-SMC4 antibody (Cat#62727). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMC4 antibody (Cat#62727, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-SMC4 antibody (Cat#62727). SMC4 expression in wild type (WT) and SMC4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMC4 antibody (Cat#62727, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of SMC4 expression in HT-1080 cells using anti-SMC4 antibody (Cat#62727, 1:2,000). Green, isotype control; red, SMC4. |
 | Immunocytochemical staining of HT-1080 cells with anti-SMC4 antibody (Cat#62727, 1:1,000). Nuclei were stained blue with DAPI; SMC4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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