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| Reactivity: | Human |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | SET and MYND domain containing 4 |
| Gene Symbol: | SMYD4 |
| Synonyms: | ZMYND21 |
| Gene ID: | 114826 |
| UniProt ID: | Q8IYR2 |
| Clone ID: | 25GB4235 |
| Immunogen: | Recombinant protein of human SMYD4 |
| Dilution: | WB 1:400-1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Predicted to enable histone deacetylase binding activity. Involved in heart development. Predicted to be active in cytoplasm and nucleus.
Images
 | Western blotting analysis using anti-SMYD4 antibody (Cat#65555). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SMYD4 antibody (Cat#65555, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-SMYD4 antibody (Cat#65555). SMYD4 expression in wild-type (WT) and SMYD4 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SMYD4 antibody (Cat#65555, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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