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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | small nuclear ribonucleoprotein polypeptide A' |
| Gene Symbol: | SNRPA1 |
| Synonyms: | Lea1; U2A' |
| Gene ID: | 6627 |
| UniProt ID: | P09661 |
| Clone ID: | 25GB3175 |
| Immunogen: | A synthesized peptide derived from human SNRPA1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables RNA binding activity. Involved in mRNA splicing, via spliceosome and spermatogenesis. Located in nuclear speck. Part of U2-type catalytic step 2 spliceosome and U2-type precatalytic spliceosome. Implicated in connective tissue disease.
Images
 | Western blotting analysis using anti-SNRPA1 antibody (Cat#61657). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SNRPA1 antibody (Cat#61657, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-SNRPA1 antibody (Cat#61657). SNRPA1 expression in wild-type (WT) and SNRPA1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SNRPA1 antibody (Cat#61657, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of SNRPA1 expression in HepG2 cells using anti-SNRPA1 antibody (Cat#61657, 1:2,000). Green, isotype control; red, SNRPA1. |
 | Immunocytochemical staining of HepG2 cells with anti-SNRPA1 antibody (Cat#61657, 1:1,000). Nuclei were stained blue with DAPI; SNRPA1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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