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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | sorting nexin 5 |
| Gene Symbol: | SNX5 |
| Gene ID: | 27131 |
| UniProt ID: | Q9Y5X3 |
| Clone ID: | 24GB2310 |
| Immunogen: | A synthesized peptide derived from human SNX5 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein functions in endosomal sorting, the phosphoinositide-signaling pathway, and macropinocytosis. This gene may play a role in the tumorigenesis of papillary thyroid carcinoma. Alternative splicing results in multiple transcript variants encoding different isoforms.
Images
 | Western blotting analysis using anti-Sorting nexin 5 antibody (Cat#62718). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Sorting nexin 5 antibody (Cat#62718, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Sorting nexin 5 antibody (Cat#62718). Sorting nexin 5 expression in wild type (WT) and Sorting nexin 5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Sorting nexin 5 antibody (Cat#62718, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Sorting nexin 5 expression in HAP-1 cells using anti-Sorting nexin 5 antibody (Cat#62718, 1:2,000). Green, isotype control; red, Sorting nexin 5. |
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