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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | SRY-box transcription factor 9 |
| Gene Symbol: | SOX9 |
| Synonyms: | CMD1; SRA1; CMPD1; SRXX2; SRXY10 |
| Gene ID: | 6662 |
| UniProt ID: | P48436 |
| Clone ID: | 24GB2000 |
| Immunogen: | A synthesized peptide derived from human SOX9 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by the gene SOX9 recognizes the sequence CCTTGAG along with other members of the HMG-box class DNA-binding proteins. It acts during chondrocyte differentiation and, with steroidogenic factor 1, regulates transcription of the anti-Muellerian hormone (AMH) gene. Deficiencies lead to the skeletal malformation syndrome campomelic dysplasia, frequently with sex reversal.
Images
 | Western blotting analysis using anti-SOX9 antibody (Cat#62760). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SOX9 antibody (Cat#62760, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-SOX9 antibody (Cat#62760). SOX9 expression in wild-type (WT) and SOX9 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SOX9 antibody (Cat#62760, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of SOX9 expression in HT-1080 cells using anti-SOX9 antibody (Cat#62760, 1:2,000). Green, isotype control; red, SOX9. |
 | Immunocytochemical staining of HT-1080 cells with anti-SOX9 antibody (Cat#62760, 1:1,000). Nuclei were stained blue with DAPI; SOX9 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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