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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG2a |
| Clonality: | Monoclonal antibody |
| Gene Name: | small RNA binding exonuclease protection factor La |
| Gene Symbol: | SSB |
| Synonyms: | La; LARP3; La/SSB |
| Gene ID: | 6741 |
| UniProt ID: | P05455 |
| Clone ID: | 24GB13175 |
| Immunogen: | Recombinant protein of human SSB |
| Dilution: | WB 1:500-1:2,500; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is involved in diverse aspects of RNA metabolism, including binding and protecting poly(U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein.
Images
 | Western blotting analysis using anti-SSB antibody (Cat#64834). Total cell lysates (30 μg for H9c2 and C2C12, 10 μg for others) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SSB antibody (Cat#64834, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-SSB antibody (Cat#64834). SSB expression in wild-type (WT) and SSB shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SSB antibody (Cat#64834, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of SSB expression in HepG2 cells using anti-SSB antibody (Cat#64834, 1:2,000). Green, isotype control; red, SSB. |
 | Immunocytochemical staining of HepG2 cells with anti-SSB associated protein antibody (Cat#64834, 1:1,000). Nuclei were stained blue with DAPI; SSB associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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