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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | signal transducer and activator of transcription 1 |
| Gene Symbol: | STAT1 |
| Synonyms: | CANDF7; IMD31A; IMD31B; IMD31C; ISGF-3; STAT91 |
| Gene ID: | 6772 |
| UniProt ID: | P42224 |
| Clone ID: | 23GB4195 |
| Immunogen: | A synthesized peptide derived from human STAT1 alpha |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the STAT protein family. In response to cytokines and growth factors, STAT family members are phosphorylated by the receptor associated kinases, and then form homo- or heterodimers that translocate to the cell nucleus where they act as transcription activators. The protein encoded by this gene can be activated by various ligands including interferon-alpha, interferon-gamma, EGF, PDGF and IL6. This protein mediates the expression of a variety of genes, which is thought to be important for cell viability in response to different cell stimuli and pathogens. The protein plays an important role in immune responses to viral, fungal and mycobacterial pathogens. Mutations in this gene are associated with Immunodeficiency 31B, 31A, and 31C.
Images
 | Western blotting analysis using anti-STAT1 antibody (Cat#61713). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-STAT1 antibody (Cat#61713, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-STAT1 antibody (Cat#61713). STAT1 expression in wild-type (WT) and STAT1 shRNA knockdown (KD) H9c2 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-STAT1 antibody (Cat#61713, 1:20,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of STAT1 expression in H9c2 cells using STAT1 antibody (Cat#61713, 1:2,000). Green, isotype control; red, STAT1. |
 | Immunocytochemical staining of H9C2 cells with STAT1 antibody (Cat#61713, 1:1,000). Nuclei were stained blue with DAPI; STAT1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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