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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG2b |
| Clonality: | Monoclonal antibody |
| Gene Name: | switching B cell complex subunit SWAP70 |
| Gene Symbol: | SWAP70 |
| Synonyms: | HSPC321; SWAP-70 |
| Gene ID: | 23075 |
| UniProt ID: | Q9UH65 |
| Clone ID: | 25GB4225 |
| Immunogen: | Recombinant protein of human SWAP70 |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables cadherin binding activity. Predicted to be involved in regulation of small GTPase mediated signal transduction. Predicted to act upstream of or within isotype switching. Located in actin cytoskeleton; cytoplasm; and plasma membrane. Is active in postsynapse.
Images
 | Western blotting analysis using anti-SWAP70 antibody (Cat#65553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SWAP70 antibody (Cat#65553, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-SWAP70 antibody (Cat#65553). SWAP70 expression in wild-type (WT) and SWAP70 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SWAP70 antibody (Cat#65553, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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