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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | TANK binding kinase 1 |
| Gene Symbol: | TBK1 |
| Synonyms: | NAK; T2K; AIARV; IIAE8; FTDALS4 |
| Gene ID: | 29110 |
| UniProt ID: | Q9UHD2 |
| Clone ID: | 24GB4650 |
| Immunogen: | A synthesized peptide derived from human NAK/TBK1 (N-term) |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The NF-kappa-B (NFKB) complex of proteins is inhibited by I-kappa-B (IKB) proteins, which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the IKB proteins by IKB kinases marks them for destruction via the ubiquitination pathway, thereby allowing activation and nuclear translocation of the NFKB complex. The protein encoded by this gene is similar to IKB kinases and can mediate NFKB activation in response to certain growth factors. The protein is also an important kinase for antiviral innate immunity response.
Images
 | Western blotting analysis using anti-TANK binding kinase 1 antibody (Cat#63237). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TANK binding kinase 1 antibody (Cat#63237, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-TANK binding kinase 1 antibody (Cat#63237). TANK binding kinase 1 expression in wild type (WT) and TANK binding kinase 1 (TBK1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TANK binding kinase 1 antibody (Cat#63237, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TANK binding kinase 1 expression in HepG2 cells using anti-TANK binding kinase 1 antibody (Cat#63237, 1:2,000). Green, isotype control; red, TANK binding kinase 1. |
 | Immunocytochemical staining of HepG2 cells with anti-TANK binding kinase 1 antibody (Cat#63237, 1:1,000). Nuclei were stained blue with DAPI; TANK binding kinase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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