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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | transcription factor 7 like 1 |
| Gene Symbol: | TCF7L1 |
| Synonyms: | TCF3; TCF-3 |
| Gene ID: | 83439 |
| UniProt ID: | Q9HCS4 |
| Clone ID: | 24GB1985 |
| Immunogen: | A synthesized peptide derived from human TCF3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the T cell factor/lymphoid enhancer factor family of transcription factors. These transcription factors are activated by beta catenin, mediate the Wnt signaling pathway and are antagonized by the transforming growth factor beta signaling pathway. The encoded protein contains a high mobility group-box DNA binding domain and participates in the regulation of cell cycle genes and cellular senescence.
Images
 | Western blotting analysis using anti-Transcription factor 7 like 1 antibody (Cat#62627). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Transcription factor 7 like 1 antibody (Cat#62627, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Transcription factor 7 like 1 antibody (Cat#62627). Transcription factor 7 like 1 expression in wild type (WT) and Transcription factor 7 like 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Transcription factor 7 like 1 antibody (Cat#62627, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Transcription factor 7 like 1 expression in HT-1080 cells using anti-Transcription factor 7 like 1 antibody (Cat#62627, 1:2,000). Green, isotype control; red, Transcription factor 7 like 1. |
 | Immunocytochemical staining of HT-1080 cells with anti-transcription factor 7 like 1 antibody (Cat#62627, 1:1,000). Nuclei were stained blue with DAPI; Transcription factor 7 like 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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