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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | transcription factor AP-2 alpha |
| Gene Symbol: | TFAP2A |
| Synonyms: | AP-2; BOFS; AP2TF; TFAP2; AP-2alpha |
| Gene ID: | 7020 |
| UniProt ID: | P05549 |
| Clone ID: | 24GB2485 |
| Immunogen: | A synthesized peptide derived from human AP2 alpha |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-TFAP2A antibody (Cat#62671). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TFAP2A antibody (Cat#62671, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-TFAP2A antibody (Cat#62671). TFAP2A expression in wild type (WT) and TFAP2A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TFAP2A antibody (Cat#62671, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TFAP2A expression in HepG2 cells using anti-TFAP2A antibody (Cat#62671, 1:2,000). Green, isotype control; red, TFAP2A. |
 | Immunocytochemical staining of HepG2 cells with anti-TFAP2A antibody (Cat#62671, 1:1,000). Nuclei were stained blue with DAPI; TFAP2A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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