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| Reactivity: | Human, Mouse |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | translocase of inner mitochondrial membrane 50 |
| Gene Symbol: | TIMM50 |
| Synonyms: | MGCA9; TIM50; TIM50L |
| Gene ID: | 92609 |
| UniProt ID: | Q3ZCQ8 |
| Clone ID: | 24GB2670 |
| Immunogen: | A synthesized peptide derived from human TIMM50 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a subunit of the TIM23 inner mitochondrial membrane translocase complex. The encoded protein functions as the receptor subunit that recognizes the mitochondrial targeting signal, or presequence, on protein cargo that is destined for the mitochondrial inner membrane and matrix. This protein may also play a role in maintaining the membrane permeability barrier, and knockdown of this gene in human cells results in the release of cytochrome c and apoptosis.
Images
 | Western blotting analysis using anti-TIMM50 antibody (Cat#62682). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TIMM50 antibody (Cat#62682, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-TIMM50 antibody (Cat#62682). TIMM50 expression in wild type (WT) and TIMM50 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TIMM50 antibody (Cat#62682, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TIMM50 expression in HAP-1 cells using anti-TIMM50 antibody (Cat#62682, 1:2,000). Green, isotype control; red, TIMM50. |
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