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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | TATA element modulatory factor 1 |
| Gene Symbol: | TMF1 |
| Synonyms: | TMF; ARA160 |
| Gene ID: | 7110 |
| UniProt ID: | P82094 |
| Clone ID: | 24GB1965 |
| Immunogen: | A synthesized peptide derived from human TMF |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables nuclear androgen receptor binding activity and nuclear receptor coactivator activity. Involved in androgen receptor signaling pathway and positive regulation of transcription by RNA polymerase II. Located in Golgi apparatus.
Images
 | Western blotting analysis using anti-TMF antibody (Cat#62805). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TMF antibody (Cat#62805, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-TMF antibody (Cat#62805). TMF expression in wild-type (WT) and TMF1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-TMF antibody (Cat#62805, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). TMF1, TATA element modulatory factor 1. |
 | Flow cytometric analysis of TMF expression in HepG2 cells using anti-TMF antibody (Cat#62805, 1:2,000). Green, isotype control; red, TMF. |
 | Immunocytochemical staining of HepG2 cells with anti-TMF antibody (Cat#62805, 1:1,000). Nuclei were stained blue with DAPI; TMF was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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