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| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | TNF receptor superfamily member 12A |
| Gene Symbol: | TNFRSF12A |
| Synonyms: | FN14; CD266; TWEAKR |
| Gene ID: | 51330 |
| UniProt ID: | Q9NP84 |
| Clone ID: | 24GB2070 |
| Immunogen: | A synthesized peptide derived from human TWEAKR |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Involved in positive regulation of extrinsic apoptotic signaling pathway and regulation of wound healing. Predicted to be located in cell surface and ruffle. Predicted to be active in plasma membrane.
Images
 | Western blotting analysis using anti-TNF receptor superfamily member 12A antibody (Cat#62645). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TNF receptor superfamily member 12A antibody (Cat#62645, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-TNF receptor superfamily member 12A antibody (Cat#62645). TNF receptor superfamily member 12A expression in wild type (WT) and TNF receptor superfamily member 12A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-TNF receptor superfamily member 12A antibody (Cat#62645, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TNF receptor superfamily member 12A expression in HT-1080 cells using anti-TNF receptor superfamily member 12A antibody (Cat#62645, 1:2,000). Green, isotype control; red, TNF receptor superfamily member 12A. |
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