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| Reactivity: | Human, Mouse |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG2b |
| Clonality: | Monoclonal antibody |
| Gene Name: | tumor protein p53 binding protein 1 |
| Gene Symbol: | TP53BP1 |
| Synonyms: | p202; 53BP1; TDRD30; p53BP1 |
| Gene ID: | 7158 |
| UniProt ID: | Q12888 |
| Clone ID: | 24GB13320 |
| Immunogen: | Recombinant protein of human TP53BP1 |
| Dilution: | WB 1:500-1:2,500 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein that functions in the DNA double-strand break repair pathway choice, promoting non-homologous end joining (NHEJ) pathways, and limiting homologous recombination. This protein plays multiple roles in the DNA damage response, including promoting checkpoint signaling following DNA damage, acting as a scaffold for recruitment of DNA damage response proteins to damaged chromatin, and promoting NHEJ pathways by limiting end resection following a double-strand break. These roles are also important during V(D)J recombination, class switch recombination and at unprotected telomeres. Alternative splicing results in multiple transcript variants encoding different isoforms.
Images
 | Western blotting analysis using anti-TP53BP1 antibody (Cat#64863). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TP53BP1 antibody (Cat#64863, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-TP53BP1 antibody (Cat#64863). TP53BP1 expression in wild-type (WT) and TP53BP1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TP53BP1 antibody (Cat#64863, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
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