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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | tropomyosin 1 |
| Gene Symbol: | TPM1 |
| Synonyms: | CMH3; TMSA; CMD1Y; LVNC9; C15orf13; HEL-S-265; HTM-alpha |
| Gene ID: | 7168 |
| UniProt ID: | P09493 |
| Clone ID: | 24GB2545 |
| Immunogen: | A synthesized peptide derived from human Tropomyosin 1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.09% Sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy and dilated cardiomyopathy 1Y.
Images
 | Western blotting analysis using anti-Tropomyosin 1 antibody (Cat#62674). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Tropomyosin 1 antibody (Cat#62674, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Tropomyosin 1 antibody (Cat#62674). Tropomyosin 1 expression in wild type (WT) and Tropomyosin 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Tropomyosin 1 antibody (Cat#62674, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Tropomyosin 1 expression in HepG2 cells using anti-Tropomyosin 1 antibody (Cat#62674, 1:2,000). Green, isotype control; red, Tropomyosin 1. |
 | Immunocytochemical staining of HepG2 cells with anti-Tropomyosin 1 antibody (Cat#62674, 1:1,000). Nuclei were stained blue with DAPI; Tropomyosin 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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