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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | TNF receptor associated protein 1 |
| Gene Symbol: | TRAP1 |
| Synonyms: | HSP75; HSP 75; HSP90L; TRAP-1 |
| Gene ID: | 10131 |
| UniProt ID: | Q12931 |
| Clone ID: | 24GB2560 |
| Immunogen: | A synthesized peptide derived from human TRAP1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a mitochondrial chaperone protein that is member of the heat shock protein 90 (HSP90) family. The encoded protein has ATPase activity and interacts with tumor necrosis factor type I. This protein may function in regulating cellular stress responses. Alternate splicing results in multiple transcript variants.
Images
 | Western blotting analysis using anti-TNF receptor associated protein 1 antibody (Cat#62739). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-TNF receptor associated protein 1 antibody (Cat#62739). TNF receptor associated protein 1 expression in wild type (WT) and TNF receptor associated protein 1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . β-Tubulin serves as a loading control. The blot was incubated with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TNF receptor associated protein 1 expression in HepG2 cells using anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:2,000). Green, isotype control; red, TNF receptor associated protein 1. |
 | Immunocytochemical staining of HepG2 cells with anti-TNF receptor associated protein 1 antibody (Cat#62739, 1:1,000). Nuclei were stained blue with DAPI; TNF receptor associated protein 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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