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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | tripartite motif containing 25 |
| Gene Symbol: | TRIM25 |
| Synonyms: | EFP; Z147; RNF147; ZNF147 |
| Gene ID: | 7706 |
| UniProt ID: | Q14258 |
| Clone ID: | 25GB2695 |
| Immunogen: | A synthesized peptide derived from human TRIM25 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein is an RNA binding protein, functions as a ubiquitin E3 ligase and is involved in multiple cellular processes, including regulation of antiviral innate immunity.
Images
 | Western blotting analysis using anti-TRIM25 antibody (Cat#61535). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM25 antibody (Cat#61535, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-TRIM25 antibody (Cat#61535). TRIM25 expression in wild-type (WT) and TRIM25 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TRIM25 antibody (Cat#61535, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TRIM25 expression in HepG2 cells using anti-TRIM25 antibody (Cat#61535, 1:2,000). Green, isotype control; red, TRIM25. |
 | Immunocytochemical staining of HepG2 cells with anti-TRIM25 antibody (Cat#61535, 1:1,000). Nuclei were stained blue with DAPI; TRIM25 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm. |
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