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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Oligoclonal antibody |
| Gene Name: | tripartite motif containing 47 |
| Gene Symbol: | TRIM47 |
| Synonyms: | GOA; RNF100 |
| Gene ID: | 91107 |
| UniProt ID: | Q96LD4 |
| Immunogen: | Recombinant protein of human TRIM47 |
| Dilution: | WB 1:500-1:2,500 FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables ubiquitin protein ligase activity. Involved in protein ubiquitination. Located in cytosol.
Images
 | Western blotting analysis using anti-TRIM47 antibody (Cat#65169). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRIM47 antibody (Cat#65169, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-TRIM47 antibody (Cat#65169). TRIM47 expression in wild-type (WT) and TRIM47 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-TRIM47 antibody (Cat#65169, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TRIM47 expression in HepG2 cells using anti-TRIM47 antibody (Cat#65169, 1:2,000). Green, isotype control; red, TRIM47. |
 | Immunocytochemical staining of HepG2 cells with anti-TRIM47 antibody (Cat#65169, 1:1,000). Nuclei were stained blue with DAPI; TRIM47 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. |
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