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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | transient receptor potential cation channel subfamily M member 7 |
| Gene Symbol: | TRPM7 |
| Synonyms: | CHAK; CHAK1; ALSPDC; LTRPC7; LTrpC-7; TRP-PLIK |
| Gene ID: | 54822 |
| UniProt ID: | Q96QT4 |
| Clone ID: | 24GB2250 |
| Immunogen: | A synthesized peptide derived from human TRPM7 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene belongs to the melastatin subfamily of transient receptor potential family of ion channels. The protein encoded by this gene is both an ion channel and a serine/threonine protein kinase. The kinase activity is essential for the ion channel function, which serves to increase intracellular calcium levels and to help regulate magnesium ion homeostasis. The encoded protein is involved in cytoskeletal organization, cell adhesion, cell migration and organogenesis. Defects in this gene are a cause of amyotrophic lateral sclerosis-parkinsonism/dementia complex of Guam. The gene may also be associated with defects of cardiac function.
Images
 | Western blotting analysis using anti-TRPM7 antibody (Cat#62634). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-TRPM7 antibody (Cat#62634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-TRPM7 antibody (Cat#62634). TRPM7 expression in wild type (WT) and TRPM7 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates . Hsp90 α serves as a loading control. The blot was incubated with anti-TRPM7 antibody (Cat#62634, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of TRPM7 expression in HepG2 cells using anti-TRPM7 antibody (Cat#62634, 1:2,000). Green, isotype control; red, TRPM7. |
 | Immunocytochemical staining of HepG2 cells with anti-TRPM7 antibody (Cat#62634, 1:1,000). Nuclei were stained blue with DAPI;TRPM7 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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