Your shopping cart is empty!
| Reactivity: | Human |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | thioredoxin domain containing 5 |
| Gene Symbol: | TXNDC5 |
| Synonyms: | HCC2; ERP46; HCC-2; STRF8; PDIA15; UNQ364; ENDOPDI |
| Gene ID: | 81567 |
| UniProt ID: | Q8NBS9 |
| Clone ID: | 25GB510 |
| Immunogen: | Recombinant protein of human TXNDC5 |
| Dilution: | WB 1:2,500-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the disulfide isomerase (PDI) family of endoplasmic reticulum (ER) proteins that catalyze protein folding and thiol-disulfide interchange reactions. The encoded protein has an N-terminal endoplasmic reticulum (ER)-signal sequence, three catalytically active thioredoxin domains and a C-terminal ER-retention sequence. Its expression is induced by hypoxia and its role may be to protect hypoxic cells from apoptosis. Alternative splicing results in multiple transcript variants. Read-through transcription also exists between this gene and the neighboring upstream BLOC1S5 gene.
Images
 | Western blotting analysis using anti-thioredoxin domain containing 5 antibody (Cat#61260). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-thioredoxin domain containing 5 antibody (Cat#61260, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-thioredoxin domain containing 5 antibody (Cat#61260). Thioredoxin domain containing 5 expression in wild type (WT) and thioredoxin domain containing 5 (TXNDC5) knockdown (KD) HSHC cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-thioredoxin domain containing 5 antibody (Cat#61260, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Thioredoxin domain containing 5 expression in HepG2 cells using anti-Thioredoxin domain containing 5 antibody (Cat# 61260, 1:2,000). Green, isotype control; red, Thioredoxin domain containing 5. |
You may also be interested in:
