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KD-Validated Thymidylate synthase Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | thymidylate synthetase |
| Gene Symbol: | TYMS |
| Synonyms: | TS; TMS; DKCD; HST422; [KD Validated] Thymidylate synthase |
| Gene ID: | 7298 |
| UniProt ID: | P04818 |
| Clone ID: | 3K6B10 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-313 of human Thymidylate synthase(NP_001062.1). |
| Dilution: | WB 1:2000-1:6000; IHC 1:100-1:500 |
| Purification Method: | Affinity purification |
| Concentration: | 1.90 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-TYMS monoclonal antibody |
Background
Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy.
Images
 | Western blot analysis of lysates from wild type (WT) and Thymidylate synthase knockdown (KD) 293T cells using [KD Validated] Thymidylate synthase Rabbit mAb (A24168) at 1:5000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KD Validated] Thymidylate synthase Rabbit mAb (A24168) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using [KD Validated] Thymidylate synthase Rabbit mAb (A24168) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using [KD Validated] Thymidylate synthase Rabbit mAb (A24168) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using [KD Validated] Thymidylate synthase Rabbit mAb (A24168) at a dilution of 1:300 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining. |
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