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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquitination factor E4A |
| Gene Symbol: | UBE4A |
| Synonyms: | E4; UFD2; UBOX2; NEDHMS |
| Gene ID: | 9354 |
| UniProt ID: | Q14139 |
| Clone ID: | 24GB1785 |
| Immunogen: | A synthesized peptide derived from human UBE4A |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a member of the U-box ubiquitin ligase family. The encoded protein is involved in multiubiquitin chain assembly and plays a critical role in chromosome condensation and separation through the polyubiquitination of securin. Autoantibodies against the encoded protein may be markers for scleroderma and Crohn's disease. A pseudogene of this gene is located on the long arm of chromosome 3. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Images
 | Western blotting analysis using anti-ubiquitination factor E4 A antibody (Cat#62639). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ubiquitination factor E4 A antibody (Cat#62639, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-ubiquitination factor E4 A antibody (Cat#62639). ubiquitination factor E4 A expression in wild type (WT) and ubiquitination factor E4 A shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-ubiquitination factor E4 A antibody (Cat#62639, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of ubiquitination factor E4 A expression in HepG2 cells using anti-ubiquitination factor E4 A antibody (Cat#62639, 1:2,000). Green, isotype control; red, ubiquitination factor E4 A. |
 | Immunocytochemical staining of HepG2 cells with anti-ubiquitination factor E4 A antibody (Cat#62639, 1:1,000). Nuclei were stained blue with DAPI; ubiquitination factor E4 A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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