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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquitin protein ligase E3 component n-recognin 5 |
| Gene Symbol: | UBR5 |
| Synonyms: | DD5; EDD; HYD; EDD1 |
| Gene ID: | 51366 |
| UniProt ID: | O95071 |
| Clone ID: | 24GB2540 |
| Immunogen: | A synthesized peptide derived from human EDD |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a progestin-induced protein, which belongs to the HECT (homology to E6-AP carboxyl terminus) family. The HECT family proteins function as E3 ubiquitin-protein ligases, targeting specific proteins for ubiquitin-mediated proteolysis. This gene is localized to chromosome 8q22 which is disrupted in a variety of cancers. This gene potentially has a role in regulation of cell proliferation or differentiation.
Images
 | Western blotting analysis using anti-UBR5 antibody (Cat#62788). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UBR5 antibody (Cat#62788, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-UBR5 antibody (Cat#62788). UBR5 expression in wild type (WT) and UBR5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UBR5 antibody (Cat#62788, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of UBR5 expression in HT-1080 cells using anti-UBR5 antibody (Cat#62788, 1:2,000). Green, isotype control; red, UBR5. |
 | Immunocytochemical staining of HT-1080 cells with anti-UBR5 antibody (Cat#62788, 1:1,000). Nuclei were stained blue with DAPI; UBR5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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