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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquitin C-terminal hydrolase L5 |
| Gene Symbol: | UCHL5 |
| Synonyms: | UCH37; CGI-70; INO80R; UCH-L5 |
| Gene ID: | 51377 |
| UniProt ID: | Q9Y5K5 |
| Clone ID: | 25GB1875 |
| Immunogen: | A synthesized peptide derived from human UCH L5 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables cysteine-type deubiquitinase activity; endopeptidase inhibitor activity; and proteasome binding activity. Involved in several processes, including positive regulation of smoothened signaling pathway; protein deubiquitination; and regulation of primary metabolic process. Located in cytosol and nuclear lumen.
Images
 | Western blotting analysis using anti-UCHL5 antibody (Cat#65519). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UCHL5 antibody (Cat#65519, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-UCHL5 antibody (Cat#65519). UCHL5 expression in wild-type (WT) and UCHL5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-UCHL5 antibody (Cat#65519, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of UCHL5 expression in HepG2 cells using anti-UCHL5 antibody (Cat#65519, 1:2,000). Green, isotype control; red, UCHL5. |
 | Immunocytochemical staining of HepG2 cells with anti-UCHL5 antibody (Cat#65519, 1:1,000). Nuclei were stained blue with DAPI; UCHL5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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