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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquinol-cytochrome c reductase, Rieske iron-sulfur polypeptide 1 |
| Gene Symbol: | UQCRFS1 |
| Synonyms: | RIP1; RIS1; RISP; UQCR5; MC3DN10 |
| Gene ID: | 7386 |
| UniProt ID: | P47985 |
| Clone ID: | 24GB2460 |
| Immunogen: | A synthesized peptide derived from human UQCRFS1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Predicted to enable oxidoreductase activity. Involved in mitochondrial respiratory chain complex III assembly and respiratory electron transport chain. Located in mitochondrion. Part of respiratory chain complex III. Implicated in mitochondrial complex III deficiency.
Images
 | Western blotting analysis using anti-UQCRFS1 antibody (Cat#62783). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-UQCRFS1 antibody (Cat#62783, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-UQCRFS1 antibody (Cat#62783). UQCRFS1 expression in wild type (WT) and UQCRFS1 shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-UQCRFS1 antibody (Cat#62783, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of UQCRFS1 expression in HepG2 cells using anti-UQCRFS1 antibody (Cat#62783, 1:2,000). Green, isotype control; red, UQCRFS1. |
 | Immunocytochemical staining of HepG2 cells with anti-UQCRFS1 antibody (Cat#62783, 1:1,000). Nuclei were stained blue with DAPI; UQCRFS1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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