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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquitin specific peptidase 5 |
| Gene Symbol: | USP5 |
| Synonyms: | ISOT |
| Gene ID: | 8078 |
| UniProt ID: | P45974 |
| Clone ID: | 24GB2480 |
| Immunogen: | A synthesized peptide derived from human USP5 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Ubiquitin (see MIM 191339)-dependent proteolysis is a complex pathway of protein metabolism implicated in such diverse cellular functions as maintenance of chromatin structure, receptor function, and degradation of abnormal proteins. A late step of the process involves disassembly of the polyubiquitin chains on degraded proteins into ubiquitin monomers. USP5 disassembles branched polyubiquitin chains by a sequential exo mechanism, starting at the proximal end of the chain (Wilkinson et al., 1995 [PubMed 7578059]).
Images
 | Western blotting analysis using anti-Ubiquitin specific peptidase 5 antibody (Cat#62732). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Ubiquitin specific peptidase 5 antibody (Cat#62732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-Ubiquitin specific peptidase 5 antibody (Cat#62732). Ubiquitin specific peptidase 5 expression in wild type (WT) and Ubiquitin specific peptidase 5 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Ubiquitin specific peptidase 5 antibody (Cat#62732, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Ubiquitin specific peptidase 5 expression in HepG2 cells using anti-Ubiquitin specific peptidase 5 antibody (Cat#62732, 1:2,000). Green, isotype control; red, Ubiquitin specific peptidase 5. |
 | Immunocytochemical staining of HepG2 cells with anti-Ubiquitin specific peptidase 5 antibody (Cat#62732, 1:1,000). Nuclei were stained blue with DAPI; Ubiquitin specific peptidase 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm. |
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