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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | ubiquitin specific peptidase 9 X-linked |
| Gene Symbol: | USP9X |
| Synonyms: | FAF; FAM; hFAM; DFFRX; FAF-X; MRX99; XLID99; MRXS99F |
| Gene ID: | 8239 |
| UniProt ID: | Q93008 |
| Clone ID: | 24GB6090 |
| Immunogen: | A synthesized peptide derived from human USP9x |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is a member of the peptidase C19 family and encodes a protein that is similar to ubiquitin-specific proteases. Though this gene is located on the X chromosome, it escapes X-inactivation. Mutations in this gene have been associated with Turner syndrome. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.
Images
 | Western blotting analysis using anti-USP9X antibody (Cat#61180). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-USP9X antibody (Cat#61180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716). |
 | Western blotting analysis using anti-USP9X antibody (Cat#61180). USP9X expression in wild-type (WT) and USP9X shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-USP9X antibody (Cat#61180, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using anti-NaQ™ ECL Substrate Kit(Cat#716). |
 | Flow cytometric analysis of USP9X expression in HepG2 cells using USP9X antibody (Cat#61180, 1:2,000). Green, isotype control; red, USP9X. |
 | Immunocytochemical staining of HepG2 cells with USP9X antibody (Cat#61180, 1:1,000). Nuclei were stained blue with DAPI; USP9X was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm. |
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