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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | VPS28 subunit of ESCRT-I |
| Gene Symbol: | VPS28 |
| Synonyms: | CIIA |
| Gene ID: | 51160 |
| UniProt ID: | Q9UK41 |
| Clone ID: | 24GB1755 |
| Immunogen: | A synthesized peptide derived from human VPS28 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a protein subunit of the ESCRT-I complex (endosomal complexes required for transport), which functions in the transport and sorting of proteins into subcellular vesicles. This complex can also be hijacked to facilitate the budding of enveloped viruses from the cell membrane. Alternative splicing results in multiple transcript variants encoding different isoforms.
Images
 | Western blotting analysis using anti-VPS28 antibody (Cat#62637). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-VPS28 antibody (Cat#62637, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-VPS28 antibody (Cat#62637). VPS28 expression in wild type (WT) and VPS28 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-VPS28 antibody (Cat#62637, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of VPS28 expression in C2C12 cells using anti-VPS28 antibody (Cat#62637, 1:2,000). Green, isotype control; red, VPS28. |
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