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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB |
| Host Species: | Mouse |
| Isotype: | IgG2a |
| Clonality: | Monoclonal antibody |
| Gene Name: | YY1 transcription factor |
| Gene Symbol: | YY1 |
| Synonyms: | DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1 |
| Gene ID: | 7528 |
| UniProt ID: | P25490 |
| Clone ID: | 24GB7020 |
| Immunogen: | Recombinant protein of human YY1 |
| Dilution: | WB 1:1,000-1:5,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
YY1 is a ubiquitously distributed transcription factor belonging to the GLI-Kruppel class of zinc finger proteins. The protein is involved in repressing and activating a diverse number of promoters. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the function of YY1.
Images
 | Western blotting analysis using anti-YY1 antibody (Cat#63709). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-YY1 antibody (Cat#63709). YY1 expression in wild-type (WT) and YY1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-YY1 antibody (Cat#63709, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
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