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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG2a |
| Clonality: | Monoclonal antibody |
| Gene Name: | FERM domain containing kindlin 2 |
| Gene Symbol: | FERMT2 |
| Synonyms: | MIG2; KIND2; mig-2; UNC112; PLEKHC1; UNC112B |
| Gene ID: | 10979 |
| UniProt ID: | Q96AC1 |
| Clone ID: | 24GB6195 |
| Immunogen: | Recombinant protein of human FERMT2 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables several functions, including phosphatidylinositol-3,4,5-trisphosphate binding activity; protein serine/threonine kinase binding activity; and type I transforming growth factor beta receptor binding activity. Involved in several processes, including cell surface receptor signaling pathway; positive regulation of cellular component biogenesis; and positive regulation of intracellular signal transduction. Acts upstream of or within cell adhesion and protein localization to cell junction. Located in several cellular components, including adherens junction; cytoplasmic side of plasma membrane; and focal adhesion. Biomarker of acute myeloid leukemia.
Images
 | Western blotting analysis using anti-FERMT2 antibody (Cat#71115). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FERMT2 antibody (Cat#71115, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716). |
 | Western blotting analysis using anti-FERMT2 antibody (Cat#71115). FERMT2 expression in wild type (WT) and FERMT2 knockout (KO) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-FERMT2 antibody (Cat#71115, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of FERMT2 expression in HepG2 cells using anti-FERMT2 antibody (Cat#71115, 1:2,000). Green, isotype control; red, FERMT2. |
 | Immunocytochemical staining of HepG2 cells with anti-FERMT2 antibody (Cat#71115, 1:1,000). Nuclei were stained blue with DAPI; FERMT2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain:Low. Scale bar, 20 μm. |
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