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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | FTO alpha-ketoglutarate dependent dioxygenase |
| Gene Symbol: | FTO |
| Synonyms: | GDFD; IFEX9; ALKBH9; BMIQ14 |
| Gene ID: | 79068 |
| UniProt ID: | Q9C0B1 |
| Clone ID: | 25GB3740 |
| Immunogen: | Recombinant protein of human FTO |
| Dilution: | WB 1:500-1:2,500; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene is a nuclear protein of the AlkB related non-haem iron and 2-oxoglutarate-dependent oxygenase superfamily but the exact physiological function of this gene is not known. Other non-heme iron enzymes function to reverse alkylated DNA and RNA damage by oxidative demethylation. Studies in mice and humans indicate a role in nervous and cardiovascular systems and a strong association with body mass index, obesity risk, and type 2 diabetes.
Images
 | Western blotting analysis using anti-FTO antibody (Cat#71249). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-FTO antibody (Cat#71249, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-FTO antibody (Cat#71249). FTO expression in wild-type (WT) and FTO knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-FTO antibody (Cat#71249, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of FTO expression in HeLa cells using anti-FTO antibody (Cat#71249, 1:2,000). Green, isotype control; red, FTO. |
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