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| Reactivity: | Human, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | glutathione S-transferase omega 1 |
| Gene Symbol: | GSTO1 |
| Synonyms: | P28; SPG-R; GSTO 1-1; GSTTLp28; HEL-S-21 |
| Gene ID: | 9446 |
| UniProt ID: | P78417 |
| Clone ID: | 25GB3120 |
| Immunogen: | A synthesized peptide derived from human GSTO1 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
The protein encoded by this gene is an omega class glutathione S-transferase (GST) with glutathione-dependent thiol transferase and dehydroascorbate reductase activities. GSTs are involved in the metabolism of xenobiotics and carcinogens. The encoded protein acts as a homodimer and is found in the cytoplasm. Three transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-glutathione S-transferase omega 1 antibody (Cat#71218). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-glutathione S-transferase omega 1 antibody (Cat#71218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-glutathione S-transferase omega 1 antibody (Cat#71218). Glutathione S-transferase omega 1 expression in wild type (WT) and glutathione S-transferase omega 1 (GSTO1) knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-glutathione S-transferase omega 1 antibody (Cat#71218, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of Glutathione S-transferase omega 1 expression in HepG2 cells using anti-Glutathione S-transferase omega 1 antibody (Cat#71218, 1:2,000). Green, isotype control; red, Glutathione S-transferase omega 1. |
 | Immunocytochemical staining of HepG2 cells with anti-Glutathione S-transferase omega 1 antibody (Cat#71218, 1:1,000). Nuclei were stained blue with DAPI; Glutathione S-transferase omega 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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