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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC |
| Host Species: | Mouse |
| Isotype: | IgG |
| Clonality: | Oligoclonal antibody |
| Gene Name: | pyruvate dehydrogenase phosphatase catalytic subunit 1 |
| Gene Symbol: | PDP1 |
| Synonyms: | PDH; PDP; PDPC; PPM2A; PPM2C; PDPC 1 |
| Gene ID: | 54704 |
| UniProt ID: | Q9P0J1 |
| Immunogen: | Recombinant protein of human PDP1 |
| Dilution: | WB 1:500-1:2,500; FC 1:2,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Pyruvate dehydrogenase (E1) is one of the three components (E1, E2, and E3) of the large pyruvate dehydrogenase complex. Pyruvate dehydrogenase kinases catalyze phosphorylation of serine residues of E1 to inactivate the E1 component and inhibit the complex. Pyruvate dehydrogenase phosphatases catalyze the dephosphorylation and activation of the E1 component to reverse the effects of pyruvate dehydrogenase kinases. Pyruvate dehydrogenase phosphatase is a heterodimer consisting of catalytic and regulatory subunits. Two catalytic subunits have been reported; one is predominantly expressed in skeletal muscle and another one is is much more abundant in the liver. The catalytic subunit, encoded by this gene, is the former, and belongs to the protein phosphatase 2C (PP2C) superfamily. Along with the pyruvate dehydrogenase complex and pyruvate dehydrogenase kinases, this enzyme is located in the mitochondrial matrix. Mutation in this gene causes pyruvate dehydrogenase phosphatase deficiency. Multiple alternatively spliced transcript variants encoding different isoforms have been identified.
Images
 | Western blotting analysis using anti-PDP1 antibody (Cat#71167). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDP1 antibody (Cat#71167, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Western blotting analysis using anti-PDP1 antibody (Cat#71167). PDP1 expression in wild type (WT) and PDP1 knockout (KO) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-PDP1 antibody (Cat#71167, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of PDP1 expression in HepG2 cells using anti-PDP1 antibody (Cat#71167, 1:2,000). Green, isotype control; red, PDP1. |
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