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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | Yes1 associated transcriptional regulator |
| Gene Symbol: | YAP1 |
| Synonyms: | YAP; YKI; COB1; YAP2; YAP-1; YAP65 |
| Gene ID: | 10413 |
| UniProt ID: | P46937 |
| Clone ID: | 25GB3280 |
| Immunogen: | A synthesized peptide derived from human YAP1 |
| Dilution: | WB 1:4,000-1:20,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a downstream nuclear effector of the Hippo signaling pathway which is involved in development, growth, repair, and homeostasis. This gene is known to play a role in the development and progression of multiple cancers as a transcriptional regulator of this signaling pathway and may function as a potential target for cancer treatment. Alternative splicing results in multiple transcript variants encoding different isoforms.
Images
 | Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#71227). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#71227, 1:20,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-phospho-YAP1 (S127) antibody (Cat#71227). Phospho-YAP1 (S127) expression in wild type (WT) and YAP1 knockout (KO) HSHC cells with 40 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-YAP1 (S127) antibody (Cat#71227, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Phospho-YAP1 (S127) expression in HepG2 cells using anti-Phospho-YAP1 (S127) antibody (Cat#71227, 1:2,000). Green, isotype control; red, Phospho-YAP1 (S127). |
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