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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | sphingomyelin phosphodiesterase 1 |
| Gene Symbol: | SMPD1 |
| Synonyms: | ASM; NPD; ASMASE; D1 |
| Gene ID: | 6609 |
| UniProt ID: | P17405 |
| Clone ID: | 2H5Q0 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 70-340 of human SMPD1(NP_000534.3). |
| Dilution: | WB 1:1000-1:3000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.04 mg/mL |
| Buffer: | PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-SMPD1 monoclonal antibody |
Background
The protein encoded by this gene is a lysosomal acid sphingomyelinase that converts sphingomyelin to ceramide. The encoded protein also has phospholipase C activity. Defects in this gene are a cause of Niemann-Pick disease type A (NPA) and Niemann-Pick disease type B (NPB). Multiple transcript variants encoding different isoforms have been identified.
Images
 | Western blot analysis of lysates from SK-MEL-28 cells, using SMPD1 Rabbit mAb (A23894) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of various lysates,using SMPD1 Rabbit mAb (A23894) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).Exposure time: 90s. |
 | Western blot analysis of lysates from wild type (WT) and SMPD4 knockout (KO) HeLa cells,using [KO Validated] SMPD1 Rabbit mAb (A23894) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
 | Western blot analysis of lysates from wild type (WT) and SMPD4 knockout (KO) HeLa cells,using SMPD1 Rabbit mAb (A23894) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).Exposure time: 90s. |
 | Immunohistochemistry analysis of paraffin-embedded Human brain using [KO Validated] SMPD1 Rabbit mAb (A23894) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
 | Immunohistochemistry analysis of paraffin-embedded Human liver using [KO Validated] SMPD1 Rabbit mAb (A23894) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining. |
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