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| Reactivity: | Human |
| Applications: | WB, FC, IC |
| Host Species: | Mouse |
| Isotype: | IgG1 |
| Clonality: | Monoclonal antibody |
| Gene Name: | syntaxin 17 |
| Gene Symbol: | STX17 |
| Gene ID: | 55014 |
| UniProt ID: | P56962 |
| Clone ID: | 24GB15925 |
| Immunogen: | Recombinant protein of human STX17 |
| Dilution: | WB 1:400-1:2,000 FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.01% thimerosal, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
Enables SNAP receptor activity; SNARE binding activity; and protein phosphatase binding activity. Involved in several processes, including autophagosome membrane docking; endoplasmic reticulum to Golgi vesicle-mediated transport; and endoplasmic reticulum-Golgi intermediate compartment organization. Acts upstream of or within protein localization to phagophore assembly site. Located in several cellular components, including autophagosome membrane; endoplasmic reticulum-Golgi intermediate compartment; and mitochondria-associated endoplasmic reticulum membrane contact site. Part of SNARE complex.
Images
 | Western blotting analysis using anti-syntaxin 17 antibody (Cat#71159). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-syntaxin 17 antibody (Cat#71159, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-syntaxin 17 antibody (Cat#71159). Syntaxin 17 expression in wild-type (WT) and syntaxin 17 (STX17) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-syntaxin 17 antibody (Cat#71159, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226). |
 | Flow cytometric analysis of Syntaxin 17 expression in HepG2 cells using anti-Syntaxin 17 antibody (Cat#71159, 1:2,000). Green, isotype control; red, Syntaxin 17. |
 | Immunocytochemical staining of HepG2 cells with anti-Syntaxin 17 antibody (Cat#71159, 1:1,000). Nuclei were stained blue with DAPI; Syntaxin 17 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm. |
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