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Reactivity: | Human, Mouse, Rat |
Applications: | WB, IP, ELISA |
Host Species: | Rabbit |
Clonality: | Polyclonal antibody |
Gene Name: | lysine demethylase 1A |
Gene Symbol: | KDM1A |
Synonyms: | AOF2; CPRF; KDM1; LSD1; BHC110 |
Gene ID: | 23028 |
UniProt ID: | O60341 |
Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 365-515 of human KDM1 (NP_055828.2). |
Dilution: | WB 1:500-1:2000 |
Purification Method: | Affinity purification |
Concentration: | 1.7mg/ml |
Buffer: | PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Documents: | Manual-KDM1A antibody |
Background
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
Images
![]() | Western blot analysis of lysates from MCF7 cells, using KDM1 Rabbit pAb (A1156) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). |
![]() | Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg KDM1 antibody (A1156). Western blot was performed from the immunoprecipitate using KDM1 antibody (A1156) at a dilution of 1:500. |
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