KDM1 Rabbit pAb (20 μl)

KDM1 Rabbit pAb (20 μl)

Brand: Abclonal
Cat. #: A1156
Availability: In Stock
$108.00
-+
Reactivity: Human, Mouse, Rat    
Applications: WB, IP, ELISA    
Host Species: Rabbit    
Clonality: Polyclonal antibody    
Gene Name: lysine demethylase 1A
Gene Symbol: KDM1A
Synonyms: AOF2; CPRF; KDM1; LSD1; BHC110
Gene ID: 23028
UniProt ID: O60341
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 365-515 of human KDM1 (NP_055828.2).
Dilution: WB 1:500-1:2000
Purification Method: Affinity purification
Concentration: 1.7mg/ml
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Storage: Store at -20°C. Avoid freeze/thaw cycles.
Documents: Manual-KDM1A antibody




Background

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.




Images

CD40 antibodyWestern blot analysis of lysates from MCF7 cells, using KDM1 Rabbit pAb (A1156) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Immunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg KDM1 antibody (A1156). Western blot was performed from the immunoprecipitate using KDM1 antibody (A1156) at a dilution of 1:500.



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