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KO-Validated CTIP1/BCL11A Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IHC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | BCL11 transcription factor A |
| Gene Symbol: | BCL11A |
| Synonyms: | EVI9; CTIP1; DILOS; ZNF856; HBFQTL5; SMARCM1; 1A |
| Gene ID: | 53335 |
| UniProt ID: | Q9H165 |
| Clone ID: | 3M8M3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 401-589 of human CTIP1/BCL11A (Q9H165). |
| Dilution: | WB 1:500-1:1000; IHC 1:50-1:200 |
| Purification Method: | Affinity purification |
| Concentration: | 1.5 mg/mL |
| Buffer: | PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-BCL11A monoclonal antibody |
Background
The gene BCL11A encodes a C2H2 type zinc-finger protein by its similarity to the mouse Bcl11a/Evi9 protein. The corresponding mouse gene is a common site of retroviral integration in myeloid leukemia, and may function as a leukemia disease gene, in part, through its interaction with BCL6. During hematopoietic cell differentiation, this gene is down-regulated. It is possibly involved in lymphoma pathogenesis since translocations associated with B-cell malignancies also deregulates its expression. Multiple transcript variants encoding several different isoforms have been found for this gene.
Images
 | Western blot analysis of lysates from wild type(WT) and CTIP1/BCL11A knockout (KO) 293T(KO) cells, using CTIP1/BCL11A Rabbit mAb (A9231) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s. |
 | Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using CTIP1/BCL11A Rabbit mAb (A9231) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining. |
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