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KO-Validated Cleaved Gasdermin D (N Terminal) Rabbit mAb (20 μl)
| Reactivity: | Human |
| Applications: | WB, IF/IC, ELISA |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | gasdermin D |
| Gene Symbol: | GSDMD |
| Synonyms: | DF5L; DFNA5L; FKSG10; GSDMDC1 |
| Gene ID: | 79792 |
| UniProt ID: | P57764 |
| Clone ID: | 3G1A6 |
| Immunogen: | A synthetic peptide corresponding to residues surrounding Asp275 of human Gasdermin D(NP_079012.3). |
| Dilution: | WB 1:70000-1:280000; IF/IC 1:100-1:400 |
| Purification Method: | Affinity purification |
| Concentration: | 0.7 mg/mL |
| Buffer: | PBS with 0.09% Sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze / thaw cycles. |
| Documents: | Manual-GSDMD monoclonal antibody |
Background
Gasdermin D is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. Gasdermin D has been suggested to act as a tumor suppressor. Alternatively spliced transcript variants have been described.
Images
 | Western blot analysis of lysates from THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059) at 1:72000 dilution incubated overnight at 4℃. THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 45s. |
 | Western blot analysis of lysates from wild type (WT) and Cleaved Gasdermin D knockout (KO) THP-1 cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059) at 1:72000 dilution incubated at room temperature for 1.5 hours. THP-1 cells and Gasdermin D knockout (KO) THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 20s. |
 | Confocal imaging of THP-1 cells (treated with PMA and LPS) and THP-1 cells (untreated) cells using [KO Validated] Cleaved Gasdermin D (N Terminal) Rabbit mAb (A24059, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x. |
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