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| Reactivity: | Human, Mouse, Rat |
| Applications: | WB, FC, IC |
| Host Species: | Rabbit |
| Isotype: | IgG |
| Clonality: | Monoclonal antibody |
| Gene Name: | serine/threonine kinase 3 |
| Gene Symbol: | STK3 |
| Synonyms: | KRS1; MST2 |
| Gene ID: | 6788 |
| UniProt ID: | Q13188 |
| Clone ID: | 25GB3265 |
| Immunogen: | A synthesized peptide derived from human STK3 |
| Dilution: | WB 1:1,000-1:5,000; FC 1:2,000; IC 1:100-1:1,000 |
| Purification Method: | Affinity purified |
| Concentration: | Lot dependent |
| Buffer: | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Storage: | Store at -20°C. Avoid freeze/thaw cycles. |
Background
This gene encodes a serine/threonine protein kinase activated by proapoptotic molecules indicating the encoded protein functions as a growth suppressor. Cleavage of the protein product by caspase removes the inhibitory C-terminal portion. The N-terminal portion is transported to the nucleus where it homodimerizes to form the active kinase which promotes the condensation of chromatin during apoptosis. Multiple transcript variants encoding different isoforms have been found for this gene.
Images
 | Western blotting analysis using anti-STK3 antibody (Cat#71226). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-STK3 antibody (Cat#71226, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Western blotting analysis using anti-STK3 antibody (Cat#71226). STK3 expression in wild type (WT) and STK3 knockout (KO) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-STK3 antibody (Cat#71226, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). |
 | Flow cytometric analysis of STK3 expression in HepG2 cells using anti-STK3 antibody (Cat#71226, 1:2,000). Green, isotype control; red, STK3. |
 | Immunocytochemical staining of HepG2 cells with anti-Phospho-YAP1 (S127) antibody (Cat#71227, 1:1,000). Nuclei were stained blue with DAPI; Phospho-YAP1 (S127) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm. |
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